List of publications Laboratory of Environmental Biotechnology


Michalíková, K., Linhartová, L., Ezechiáš, M., Cajthaml, T. (2019) Assessment of agonistic and antagonistic properties of widely used oral care antimicrobial substances toward steroid estrogenic and androgenic receptors. Chemosphere, 217, 534-541. Article link

Ezechiáš, M., Cajthaml, T. (2019) Estimation of competitive antagonist affinity by the Schild method and from functional inhibition curves using a novel form of the Gaddum equation. Toxicology, 420, 21-28. Article link

Palyzová, A., Svobodová, K., Sokolová, L., Novák, J., Novotný, Č. (2019) Metabolic profiling of Fusarium oxysporum f. sp. conglutinans race 2 in dual cultures with biocontrol agents Bacillus amyloliquefaciens, Pseudomonas aeruginosa, and Trichoderma harzianum. Folia Microbiologica, In press. Article link


Semerád, J., Čvančarová, M., Filip, J., Kašlík, J., Zlotá, J., Soukupová, J., Cajthaml, T. (2018) Novel assay for the toxicity evaluation of nanoscale zero-valent iron and derived nanomaterials based on lipid peroxidation in bacterial species. Chemosphere, 213, 568-577. Article link

Innemanová, P., Filipová, A., Michalíková, K., Wimmerová, L., Cajthaml, T. (2018) Bioaugmentation of PAH-contaminated soils: A novel procedure for introduction of bacterial degraders into contaminated soil. Ecological Engineering, 118, 93-96. Article link

Křesinová, Z., Linhartová, L., Filipová, A., Ezechiáš, M., Mašín, P., Cajthaml, T. (2018) Biodegradation of endocrine disruptors in urban wastewater using Pleurotus ostreatus bioreactor. New Biotechnology, 43, 53-61. Article link

Ezechiáš, M., Cajthaml, T. (2018) New insight into isobolographic analysis for combinations of a full and partial agonist: Curved isoboles. Toxicology, 402-403, 9-16. Article link

Ezechiáš, M., Cajthaml, T. (2018) Receptor partial agonism and method to express receptor partial activation with respect to novel Full Logistic Model of mixture toxicology. Toxicology, 393, 26-33. Article link

Novotny, C., Schulzova, V., Krmela, A., Hajslova, J., Svobodova, K., Koudela, M. (2018) Ascorbic acid and glucosinolate levels in new czech cabbage cultivars: Effect of production system and fungal infection. Molecules, 23, Article number 1855. Article link

Svobodova, K., Novotny, C. (2018) Bioreactors based on immobilized fungi: bioremediation under non-sterile conditions. Applied Microbiology and Biotechnology, 102, 39-46. Article link

Svobodova, K., Semerad, J., Petrackova, D., Novotny, C. (2018) Antibiotic Resistance in Czech Urban Wastewater Treatment Plants: Microbial and Molecular Genetic Characterization. Microbial Drug Resistance, 24, 830-838. Article link


Lhotský, O., Krákorová, E., Linhartová, L., Křesinová, Z., Steinová, J., Dvořák, L., Rodsand, T., Filipová, A., Kroupová, K., Wimmerová, L., Kukačka, J., Cajthaml, T. (2017) Assessment of biodegradation potential at a site contaminated by a mixture of BTEX, chlorinated pollutants and pharmaceuticals using passive sampling methods – Case study. Science of the Total Environment, 607-608, 1451-1465.

Lhotský, O., Krákorová, E., Mašín, P., Žebrák, R., Linhartová, L., Křesinová, Z., . . . Cajthaml, T. (2017). Pharmaceuticals, benzene, toluene and chlorobenzene removal from contaminated groundwater by combined UV/H2O2 photo-oxidation and aeration. Water Research, 120, 245-255.

Kringberg, H.B., RØdsand, T., Telstad, R.S., Lhotsky, O., Krakorova, E., Cajthaml, T. (2017) Passive sampling approaches and their applicability for contaminated sites monitoring. Waste Forum (1), 5-16.

Siles, J. A., Cajthaml, T., Filipová, A., Minerbi, S., & Margesin, R. (2017). Altitudinal, seasonal and interannual shifts in microbial communities and chemical composition of soil organic matter in alpine forest soils. Soil Biology and Biochemistry, 112, 1-13.

Stella, T., Covino, S., Čvančarová, M., Filipová, A., Petruccioli, M., D’Annibale, A., & Cajthaml, T. (2017). Bioremediation of long-term PCB-contaminated soil by white-rot fungi. Journal of Hazardous Materials, 324, 701-710.

Stella T, Covino S, Čvančarová M, Filipová A, Petruccioli M, D’Annibale A, Cajthaml T. (2017) Bioremediation of long-term PCB-contaminated soil by white-rot fungi. Journal of Hazardous Materials, 324, 701-10.


Covino S, Fabianová T, Křesinová Z, Čvančarová M, Burianová E, Filipová A, Voříšková J, Baldrian P, Cajthaml T. (2016) Polycyclic aromatic hydrocarbons degradation and microbial community shifts during co-composting of creosote-treated wood. Journal of Hazardous Materials, 301, 17-26.

Covino S, Stella T, D’Annibale A, Lladó S, Baldrian P, Čvančarová M, Cajthaml T, Petruccioli M. (2016) Comparative assessment of fungal augmentation treatments of a fine-textured and historically oil-contaminated soil. Sci. Total. Environ., 566-567, 250-9.

Ezechiáš M, Cajthaml T. (2016) Novel full logistic model for estimation of the estrogenic activity of chemical mixtures. Toxicology, 359-360, 58-70.

Ezechiáš M, Janochová J, Filipová A, Křesinová Z, Cajthaml T. (2016) Widely used pharmaceuticals present in the environment revealed as in vitro antagonists for human estrogen and androgen receptors. Chemosphere, 152, 284-91.

Křesinová Z, Linhartová L, Petrů K, Krejčová L, Šrédlová K, Lhotský O, Kameník Z, Cajthaml T. (2016) Method for analysis of psychopharmaceuticals in real industrial wastewater and groundwater with suspended organic particulate matter using solid phase extraction disks extraction and ultra-high performance liquid chromatography/time-of-flight mass spectrometry. J Chromatogr A, 1440, 15-22.

Křesinová Z, Petrů K, Lhotský O, Rodsand T, Cajthaml T. (2016) Passive sampling of pharmaceuticals and personal care products in aquatic environments. European Journal of Environmental Science, 6;43:56.

Semerád J, Cajthaml T. (2016) Ecotoxicity and environmental safety related to nano-scale zerovalent iron remediation applications. Applied Microbiology and Biotechnology, 100(23), 9809-9819.



Cajthaml T. Biodegradation of endocrine-disrupting compounds by ligninolytic fungi: Mechanisms involved in the degradation. Environ Microbiol 2015;17(12):4822-34.

Covino S, D’Annibale A, Stazi SR, Cajthaml T, Čvančarová M, Stella T, et al. Assessment of degradation potential of aliphatic hydrocarbons by autochthonous filamentous fungi from a historically polluted clay soil. Sci Total Environ. 2015;505:545-54.

Čvančarová M, Cajthaml T. Methods for determination of bioaccessibility and bioavailability of hydrophobic organic pollutants. Chem Listy 2015;109(4):254-68.

Stella T, Covino S, Burianová E, Filipová A, Křesinová Z, Voříšková J, Větrovský T, Baldrian P, Cajthaml T. Chemical and microbiological characterization of an aged PCB-contaminated soil. Sci Total Environ 2015;533:177-86.


Cajthaml T. Biodegradation of endocrine-disrupting compounds by ligninolytic fungi: Mechanisms involved in the degradation. Environ Microbiol. 2014.

Čvančarová M, Moeder M, Filipová A, Cajthaml T. Biotransformation of fluoroquinolone antibiotics by ligninolytic fungi – metabolites, enzymes and residual antibacterial activity. Chemosphere 2014;136:311-20.

Ezechiáš M, Covino S, Cajthaml T. Ecotoxicity and biodegradability of new brominated flame retardants: A review. Ecotoxicol Environ Saf. 2014;110:153-67.

Křesinová Z, Hostačná L, Medková J, Čvančarová M, Stella T, Cajthaml T. Sensitive GC/MS determination of 15 isomers of chlorobenzoic acids in accelerated solvent extracts of soils historically contaminated with PCBs and validation of the entire method. Int J Environ Anal Chem. 2014;94(8):822-36.

Němeček J, Lhotský O, Cajthaml T. Nanoscale zero-valent iron application for in situ reduction of hexavalent chromium and its effects on indigenous microorganism populations. Sci Total Environ. 2014;485-486(1):739-47.


Čvančarová M, Moeder M, Filipová A, Reemtsma T, Cajthaml T. Biotransformation of the antibiotic agent flumequine by ligninolytic fungi and residual antibacterial activity of the transformation mixtures. Environmental Science and Technology. 2013;47(24):14128-36.

Čvančarová M, Křesinová Z, Cajthaml T. Influence of the bioaccessible fraction of polycyclic aromatic hydrocarbons on the ecotoxicity of historically contaminated soils. J Hazard Mater. 2013;254-255(1):116-24.

Přenosilová L, Křesinová Z, Amemori AS, Cajthaml T, Svobodová K. Transcriptional response of lignin-degrading enzymes to 17α-ethinylestradiol in two white rots. Microbial Biotechnology. 2013;6(3):300-6.

Stella T, Covino S, Křesinová Z, D’Annibale A, Petruccioli M, Čvančarová M, et al. Chlorobenzoic acid degradation by Lentinus (Panus) tigrinus: In vivo and in vitro mechanistic study-evidence for P-450 involvement in the transformation. J Hazard Mater. 2013;260:975-83.


Čvančarová M, Křesinová Z, Filipová A, Covino S, Cajthaml T. Biodegradation of PCBs by ligninolytic fungi and characterization of the degradation products. Chemosphere. 2012;88(11):1317-23.

Ezechiáš M, Svobodová K, Cajthaml T. Hormonal activities of new brominated flame retardants. Chemosphere. 2012;87(7):820-4.

Křesinová Z, Moeder M, Ezechiáš M, Svobodová K, Cajthaml T. Mechanistic study of 17α-ethinylestradiol biodegradation by Pleurotus ostreatus: Tracking of extracelullar and intracelullar degradation mechanisms. Environmental Science and Technology. 2012;46(24):13377-85.

Plačková M, Svobodová K, Cajthaml T. Laccase activity profiling and gene expression in PCB-degrading cultures of Trametes versicolor. International Biodeterioration and Biodegradation. 2012;71:22-8.


Federici E, Giubilei MA, Cajthaml T, Petruccioli M, D’Annibale A. Lentinus (Panus) tigrinus augmentation of a historically contaminated soil: Matrix decontamination and structure and function of the resident bacterial community. J Hazard Mater. 2011;186(2-3):1263-70.

Křesinová Z, Muzikář M, Olšovská J, Cajthaml T. Determination of 15 isomers of chlorobenzoic acid in soil samples using accelerated sample extraction followed by liquid chromatography. Talanta. 2011;84(4):1141-7.

Muzikář M, Křesinová Z, Svobodová K, Filipová A, Čvančarová M, Cajthamlová K, et al. Biodegradation of chlorobenzoic acids by ligninolytic fungi. J Hazard Mater. 2011;196:386-94.


Covino S, Čvančarová M, Muzikář M, Svobodová K, D’annibale A, Petruccioli M, et al. An efficient PAH-degrading Lentinus (Panus) tigrinus strain: Effect of inoculum formulation and pollutant bioavailability in solid matrices. J Hazard Mater. 2010;183(1-3):669-76.

Covino S, Svobodová K, Křesinová Z, Petruccioli M, Federici F, D’Annibale A, et al. In vivo and in vitro polycyclic aromatic hydrocarbons degradation by Lentinus (Panus) tigrinus CBS 577.79. Bioresour Technol. 2010;101(9):3004-12.

Covino S, Svobodová K, Čvančarová M, D’Annibale A, Petruccioli M, Federici F, et al. Inoculum carrier and contaminant bioavailability affect fungal degradation performances of PAH-contaminated solid matrices from a wood preservation plant. Chemosphere. 2010;79(8):855-64.

Hubálek T, Čvančarová M, Cajthaml T. Ecotoxicity of PAH-contaminated soil during composting. Acta Universitatis Carolinae, Environmentalica. 2010;24(1-2):51-60.

Svobodová K, Cajthaml T. New in vitro reporter gene bioassays for screening of hormonal active compounds in the environment. Appl Microbiol Biotechnol. 2010;88(4):839-47.


Cajthaml T, Křesinová Z, Svobodová K, Sigler K, Řezanka T. Microbial transformation of synthetic estrogen 17α-ethinylestradiol. Environmental Pollution. 2009;157(12):3325-35.

Křesinová Z, Svobodová K, Cajthaml T. Microbial degradation of endocrine disruptors. Chem Listy. 2009;103(3):200-7.

Olšovská J, Kameník Z, Cajthaml T. Hyphenated ultra high-performance liquid chromatography-nano quantity analyte detector technique for determination of compounds with low UV absorption. Journal of Chromatography A. 2009;1216(30):5774-8.

Novotný C, Cajthaml T, Svobodová K, Šušla M, Šašek V. Irpex lacteus, a white-rot fungus with biotechnological potential – review. Folia Microbiol (Praha). 2009;54(5):375-90.

Svobodová K, Plačková M, Novotná V, Cajthaml T. Estrogenic and androgenic activity of PCBs, their chlorinated metabolites and other endocrine disruptors estimated with two in vitro yeast assays. Sci Total Environ. 2009;407(22):5921-5.

Cajthaml T, Křesinová Z, Svobodová K, Möder M. Biodegradation of endocrine-disrupting compounds and suppression of estrogenic activity by ligninolytic fungi. Chemosphere. 2009;75(6):745-50.


Baborová P, Möder M, Baldrian P, Cajthamlová K, Cajthaml T. Purification of a new manganese peroxidase of the white-rot fungus Irpex lacteus, and degradation of polycyclic aromatic hydrocarbons by the enzyme. Res Microbiol 2006;157(3):248-53.
Bhatt M, Cajthaml T, Šašek V. Mycoremediation of PAH-contaminated soil. Folia Microbiol 2002;47(3):255-8.
Cajthaml T, Bhatt M, Šašek V, Matějů V. Bioremediation of PAH-contaminated soil by composting: A case study. Folia Microbiol 2002;47(6):696-700.
Cajthaml T, Erbanová P, Kollmann A, Novotný Č, Šašek V, Mougin C. Degradation of PAHs by ligninolytic enzymes of Irpex lacteus. Folia Microbiol (Praha). 2008;53(4):289-94.
Cajthaml T, Erbanová P, Šašek V, Moeder M. Breakdown products on metabolic pathway of degradation of benz[a]anthracene by a ligninolytic fungus. Chemosphere 2006;64(4):560-4.
Cajthaml T, Möder M, Kačer P, Šašek V, Popp P. Study of fungal degradation products of polycyclic aromatic hydrocarbons using gas chromatography with ion trap mass spectrometry detection. J Chromatogr A 2002;974(1-2):213-22.
Cajthaml T, Pacáková V, Šašek V. Microbial degradation of polycyclic aromatic hydrocarbons. Chem Listy 2001;95(7):404-10.
Cajthaml T, Šašek V. Application of supercritical fluid extraction (SFE) to predict bioremediation efficacy of long-term composting of PAH-contaminated soil. Environ Sci Technol 2005;39(21):8448-52.
Leonardi V, Šašek V, Petruccioli M, D’Annibale A, Erbanová P, Cajthaml T. Bioavailability modification and fungal biodegradation of PAHs in aged industrial soils. International Biodeterioration and Biodegradation. 2007;60(3):165-70.
Möder M, Cajthaml T, Schrader S, Šašek V. Solid phase microextraction (SPME) used for direct fruit-body sampling in comparison with ASE-GC-MS to describe PAH partitioning in mushroom cultures. Fresenius Environ Bull 2002;11(6):284-8.
Moeder M, Cajthaml T, Koeller G, Erbanová P, Šašek V. Structure selectivity in degradation and translocation of polychlorinated biphenyls (delor 103) with a Pleurotus ostreatus (oyster mushroom) culture. Chemosphere 2005;61(9):1370-8
Novotný Č, Erbanová P, Cajthaml T, Rothschild N, Dosoretz C, Šašek V. Irpex lacteus, a white rot fungus applicable to water and soil bioremediation. Appl Microbiol Biotechnol 2000;54(6):850-3.
Novotný Č, Erbanová P, Šašek V, Kubátová A, Cajthaml T, Lang E, Krahl J, Zadražil F. Extracellular oxidative enzyme production and PAH removal in soil by exploratory mycelium of white rot fungi. Biodegradation 1999;10(3):159-68.
Novotný Č, Svobodová K, Erbanová P, Cajthaml T, Kasinath A, Lang E, Šašek V. Ligninolytic fungi in bioremediation: Extracellular enzyme production and degradation rate. Soil Biol Biochem 2004;36(10):1545-51.
Sasek V, Novotny C, Erbanova P, Bhatt M, Cajthaml T, Kubatova A. Selection of ligninolytic fungi for biodegradation of organopollutants. Batelle Meml Inst Int in Situ on Site Bioreclam Symp Proc 1999;5.
Šašek V, Bhatt M, Cajthaml T, Malachová K, Lednická D. Compost-mediated removal of polycyclic aromatic hydrocarbons from contaminated soil. Arch Environ Contam Toxicol 2003;44(3):336-42.
Šašek V, Cajthaml T, Bhatt M. Use of fungal technology in soil remediation: A case study. Water Air Soil Pollut Focus 2003;3(3):5-14.
Tuháčková J, Cajthaml T, Novák K, Novotný, Mertelík J, Šašek V. Hydrocarbon deposition and soil microflora as affected by highway traffic. Environ Pollut 2001;113(3):255-62.


Book Chapters

Cajthaml T, Svobodová K. Biodegradation of aromatic pollutants by ligninolytic fungal strains. In: Microbial biodegradation of xenobiotics. Springer-Verlag 2012. p. 291-316.

Covino S, Stella T, Cajthaml T. Mycoremediation of organic pollutants: Principles, opportunities, and pitfalls. In: Fungal Applications in Sustainable Environmental Biotechnology. Springer 2016. p.85-231.

Křesinová Z, Žebrák R, Langová J, Cajthaml T. Assessment of the presence of triclosan in wastewater treatment plants in the Czech Republic and alternative fungal treatment. In: Wastewater treatment: Processes, management strategies and Environmental/Health impacts. Nova Publishing 2015. p 69-79.

České publikace a vědecko-populární publikace

Cajthaml T, Křesinová Z. Hormonální látky kolem nás: hrozba 21. století. Výzkumný program Rozmanitost života a zdraví ekosystémů. Strategie AV 21, Akademie věd České Republiky, 2016.

Křesinová Z, Cajthaml T. Nová typy polutantů – endokrinní disruptory  v životním prostředí a jejich biodegradace. In: Příležitosti výzvy environmentálního výzkumu (Jan Frouz a Bedřich Moldan). Karolinum 2015. Str.:86-106.